13 new Canola Agronomic Research Program Projects
Integrated pest management
DISEASES
Project: Biocontrol of blackleg using carnivorous bacteria (part 2)
Principal investigator: Paul Holloway, University of Winnipeg
Funding: Manitoba Canola Growers, Western Grains Research Foundation
Purpose: To identify strains and species of bacteria from Manitoban soils that inhibit/kill blackleg, challenge bacteria-inoculated seedlings with blackleg and identify if the same bacteria are effective against verticillium stripe.
Project: Methods to isolate and maintain clubroot for improved resistance screening and labelling
Principal investigator: Stephen Strelkov, University of Alberta
Funding: Alberta Canola, Manitoba Canola Growers, SaskOilseeds, Western Grains Research Foundation
Purpose: To develop best practices to maintain clubroot isolates on plants, optimize micro-lasers to isolate single spores and work with the Clubroot Steering Committee to introduce clubroot labelling on major pathotypes.
Project: In vitro culture of Plasmodiophora brassicae
Principal investigator: Mary Ruth McDonald, University of Guelph
Funding: Alberta Canola, SaskOilseeds, Western Grains Research Foundation
Purpose: To develop a method for in vitro culturing of clubroot and produce single-spore cultures of clubroot for sequencing.
Project: Clubroot pathotype evaluation and monitoring
Principal investigator: Stephen Strelkov, University of Alberta
Funding: Alberta Canola, SaskOilseeds, Western Grains Research Foundation
Purpose: Tracking clubroot occurrence, severity and spread, generating isolates from collected root samples, and monitoring pathotype composition, virulence shifts and potential resistance breaking pathotypes.
Project: Expanding BnVQs (Valene-Glutamine) gene family against Sclerotinia sclerotiorum in canola
Principal investigator: Zhongwei Zou, Wilfred Laurier University
Funding: SaskOilseeds, Western Grains Research Foundation
Purpose: To evaluate sclerotinia resistance of canola varieties (spring canola, winter canola, blackleg resistance lines) and characterize BnVQ gene expression patterns. Produce transgenic lines overexpressing critical BnVQ genes and identify novel genes that can improve resistance.
Project: Investigating the conditions favouring verticillium stripe development and yield losses in canola
Principal investigator: Sheau-Fang Hwang, University of Alberta and Fouad Daayf, University of Manitoba
Funding: Alberta Canola, Manitoba Canola Growers, SaskOilseeds, Western Grains Research Foundation
Purpose: To examine interactions between verticillium stripe and blackleg in soil and in lab, evaluate the impact of pH on verticillium stripe, investigate how canola defenses react to verticillium stripe and determine the lineage/specificity of collected isolates while also looking at the verticillium stripe seed infection rate.
Project: Comparative analysis of Verticillium longisporum lineages in the Canadian Prairies: Safeguarding canola production
Principal investigator: Zhongwei Zou, Wilfred Laurier University and Harmeet Singh Chawla, University of Manitoba
Funding: SaskOilseeds, Western Grains Research Foundation
Purpose: Collect and characterize different V. longisporum isolates prevalent in the Prairies to provide critical genetics and genomics knowledge on verticillium stripe and the disease progression in canola plants.
Project: A comprehensive survey of verticillium stripe and establishment of a disease nursery in Morden, Manitoba
Principal investigator: Ahmed Abdelmagid, Agriculture and Agri-Food Canada Morden
Funding: Manitoba Canola Growers, SaskOilseeds, Western Grains Research Foundation
Purpose: To conduct a survey across Manitoba to build a collection of isolates to develop a disease breeding nursery in Morden, and to characterize the lineages of collected isolates.
Project: Impact of synergistic interaction between Verticillium longisporum and Leptosphaeria maculans on canola yield
Principal investigator: Hossein Borhan, Agriculture and Agri-Food Canada Saskatoon
Funding: Alberta Canola, SaskOilseeds, Western Grains Research Foundation
Purpose: To investigate the compound impact of verticillium stripe and blackleg on susceptible canola, identify sources of resistance to verticillium stripe, test durability of multiple gene blackleg resistance lines and develop susceptible/resistance check lines to test verticillium stripe resistance.
INSECTS
Project: Population dynamics and monitoring programs for midges attacking canola
Principal investigator: Meghan Vankosky, Agriculture and Agri-Food Canada, Saskatoon
Funding: Alberta Canola, SaskOilseeds, Western Grains Research Foundation
Purpose: The utilization of pheromone traps to monitor swede midge and canola flower midge for early detection of invasion and monitor population density/geographic range. Also to determine growing season population dynamics of flower midge and its potential impact on yields.
Project: Tracking the movement of flea beetles across the Canadian Prairies
Principal investigator: Boyd Mori, University of Alberta
Funding: Alberta Canola, Manitoba Canola Growers, SaskOilseeds
Purpose: To survey flea beetle populations across the Prairies to determine species composition and movement of beetles and to conduct a field level study to determine if flea beetles with non-crop hosts contribute to populations in nearby canola crops.
Project: Volatile-based trapping and management of flea beetles
Principal investigator: Maya Evenden, University of Alberta
Funding: Alberta Canola and SaskOilseeds
Purpose: To compare commercial flea beetle attractants and traps in spring and fall, and identify best practices for implementing lures/traps to develop a attract-and-kill formulation.
GENETICS
Project: New pre-breeding tools for canola – facilitating canola improvement by accessing diploid variation
Principal investigator: Steve Robinson, Agriculture and Agri-Food Canada, Saskatoon
Funding: SaskOilseeds, Western Grains Research Foundation
Purpose: To access new genetic variation from diploid Brassica species by marker-assisted introduction of alleles, remove reproductive barriers between B. rapa and B. oleracea and generate synthetic B. napus lines for canola breeding. The genetic resources developed during this project can be made available to the canola industry supporting additional efforts to introduce necessary variation required by canola breeders.